Membres :
Daniel
Abergel
Geoffrey
Bodenhausen
Ludovic
Carlier
Isabelle Correia
Luminita
Duma
Fabien
Ferrage
Lucie
Khemtemourian
Olivier Lequin
Emeric Miclet
Philippe
Pelupessy
Piotr
Tekely
-
Doctorants, postdoc, CDD:
Lucie Caillon
Paolo Calligari
Cyril Charlier
Monique Chan-Huot
Anais Hoffmann
A
Mamnikam Sadasivam
Khan Shahid
Akansha Sehgal
Bruno Vitorge
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Equipe 3: Structure et Dynamique des
Biomolécules
Res G Bodenhausen
ED:
ED 387 :
iViv : Interdisciplinaire pour le vivant
ED 388 :
Chimie physique et chimie analytique de Paris Centre
Our research group is associated with the Ecole Normale Supérieure and
Pierre and Marie Curie University in Paris (France). We are attached to
the Laboratory of Biomolecules - a research unit of the Centre National
de la Recherche Scientifique. We stay in a close collaboration with the
NMR group from Ecole Polytechnique Federale de Lausanne. Our group is
specialised in liquid state (internal dynamics in
biomolecules, fast proton exchange rates, cross-correlated relaxation
and single scan multidimensional experiments) and solid state (for
example, de- and recoupling and measurements of chemical shift
anisotropies) NMR method development. We also have the experience in
protein expression and purification and in magnetic resonance imaging
(MRI).
Many chemists are familiar with nuclear magnetic resonance (NMR) as a
simple analytical method that allows them to determine the composition,
constitution and conformation of molecules. Biologists often perceive
NMR as a rather laborious alternative to X-ray diffraction for the
determination of protein structures. Some physicists like to think of
nuclear spins as prototypical two-level quantum systems. Very few seem
to be aware of the breadth and variety of applications of NMR to
solids, liquids and gases. The amazing achievements of magnetic
resonance imaging (MRI) are widely known, but very few understand the
underlying principles. Our research group is working on various
fundamental and applied aspects of NMR. Some applications are too
demanding to be solved by current state-of-the-art methods, so that we
may have to develop new methods before a particular problem can be
tackled in a satisfactory manner. We shall give some typical examples
of our work in last few years:
- # the characterization of fast exchange of
labile
protons in functional
groups such as amines (with rates of ca. 100 000 jumps per second),
- # the measurement of slow rotations of aromatic
rings
embedded in proteins (on the order of 1 ring-flip per second),
- # the slow rearrangements of ribonucleic acids
(RNA’s) between different possible folds (a few inter-conversions per
second),
- # the determination of diffusion coefficients
of
macromolecules in solution (on the order of 10 -11 m2s-1, which amounts
to displacements of a few micrometers per second),
- # the study of the internal mobility of
backbones and
side-chains in proteins (typically from 10 2 to 10 6 events per
second),
- # the characterization of motions on a broad
range of
time-scales in polycrystalline samples of biomolecules (up to 10 7
jumps per second),
- # the enhancement of weak NMR signals by
dynamic
nuclear polarization (DNP), both in liquids by the so-called
‘dissolution’ method and in solids by ‘in situ’ saturation of electron
spin resonance,
- # the improvement of the spectral resolution in
solid-state NMR spectra of nuclei such as carbon-13 by magic angle
spinning (MAS),
- # the narrowing of carbon-13 signals through
proton
decoupling methods that can be combined with fast MAS,
- # the determination of the lengths and geometry
of
hydrogen and halogen bonds,
- # the indirect detection of nitrogen-14 NMR in
solids
in view of determining second-order quadrupolar couplings and the
effects of motions on these parameters,
- # the art of recording two-dimensional spectra
in a
fraction of a second, in view of monitoring exchange processes in real
time.
Grants
- Equipements d'Excellence (2011-2018): major grant (5 million Eur) for
the purchase of a 800 MHz wide-bore (WB) system equipped with a
gyrotron for solid-state DNP and high-field MRI (PI: G. Bodenhausen,
heading a consortium with 9 partners in Paris-centre and Orléans.)
- ERC Starting Grant 2012-2016: 2F4BIODYN,
Two-field NMR spectroscopy for the exploration of biomolecular dynamics
(PI: F. Ferrage)
- ANR Jeune Chercheur 2012: COLD (PI: E. Miclet)
- ANR Blanc 2012-2015: DYN-IDP, Proteins: the timescale of disorder (PI: F. Ferrage)
- ANR Blanc 2011-2014: FT-ICR 2D, Two-dimensional mass spectrometry (PI: Ch. Rolando)
- ANR Blanc 2007-2010: SAACoude, Aminoacides à conformation contrôlée:
nouvelles approches stéréosélectives et application à la conception de
librairies de coudes bêta (Coordinator: J. Szymoniak)
- ANR Blanc 2009-2012: FastSpinProts, Jiggling
and wigglings proteins: characterization by solid-state NMR with fast
magic angle spinning (PI: G. Bodenhausen)
- ANR Blanc 2011-2013: ParaHP, Paracrine activity
and translocation of homeoproteins (Coordinator: A. Joliot,
correspondant team 3 O. Lequin)
- ANR Blanc 2011-2013: FAC, Foldzymes, foldamers
targeting catalytic activity (PI: P. Karoyan team 1, correspondant team
3 O. Lequin)
- ANR MI2 2011-2012: IMPRO-Fprau,
Immuno-modulatory effects mediated by peptides derived from a specific
(PI: P. Seksik team 4, correspondant team 3 O. Lequin)
- Convergence UPMC 2011-2012: Peptides antimicrobiens et leishmaniose (Coordinator: A. Ladram)
- Emergence UPMC 2009: RMN de protéines dans les
cellules vivantes: application au facteur de transcription Engrailed
(PI: O. Lequin)
- PGG Project 2009-2011, Magnetic resonance in inhomogeneous fields: MRS and MRI from in vitro to in vivo (PI: P. Pelupessy)
- Grant from IBISA for high-field MRI (PI: G. Bodenhausen)
Publications recentes :
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