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Accueil du site > Séminaires, conférences > Séminaires du LIP > Séminaires en 2015-2016 > Frédéric Padilla (Labtau, Lyon), invité par P. Laugier

Frédéric Padilla (Labtau, Lyon), invité par P. Laugier

Orateur : Frédéric Padilla (Labtau, Lyon)

Titre : A Bio-Acoustic Levitational (BAL) Assembly Method for Engineering 3D Multilayer Neuronal Networks

Date : Vendredi 11 Mars 2016, à 14h00

Lieu : salle 114 – bâtiment 91 (Site Pitié-Salpêtrière)

Résumé : Bioengineering aspires to build biofidelic, 3-D surrogates of native organs and tissues that are invaluable tools to examine their physical properties and functions. This is particularly relevant to neuroscience where unparalleled complexity of the human brain and limited access to human tissue are hindering progress in deciphering cell and molecular mechanisms operating during development and numerous pathologies. Current strategies mostly utilize 2D monolayer platforms to reveal the neuron cell interactions, which poorly represent the native spatial organization and functionality. Recently developed alternative based on 3D bioprinting approaches are very promising, but still rely on complex cell manipulation and require a high level of expertise. With the goal to propose a technological platform that would be easy to implement in biological labs, we recently developed a method to simply and rapidly generate multilayer tissue construct using bulk acoustic levitation. We developed an acoustic bioprinting platform allowing fabrication of 3D multilayer of human neural progenitor cells (NPC) in a simple and rapid way. NPCs are driven to nodes of acoustic standing waves by acoustic radiation force and levitated to form multilayers in less than 10 seconds within a fibrin hydrogel. Levitated NPCs are directly differentiated into neurons in 3D microenvironment and form interlayer neuronal connections. This acoustic bioprinting technology to generate multilayered neural constructs holds great potential to be utilized in neural tissue engineering applications, aiming at the development of biological relevant in vitro models of simplified laminar cortex, and also at the fabrication of multi-layered tissue models as an alternative for animal studies or brain slices. In the second part of the talk, we will present our recent progress on ultrasound-based transfection of brain stem cells. We aim at the development of an ultrasound-based method to transfect cortex cells in situ, to allow labeling of progenitors for tracking cell progression during brain development. Our first data show that using acoustic cavitation, cortex cells can be transfected in vitro with a GFP-plasmid. The achieved transfection efficiency was sufficient to track individual cells in time-lapse videomicroscopy and to analyze their migration and proliferation. Current work now aims at optimizing the ultrasound setup for applications in small animal. Reference Bouyer, C. et al. A Bio-Acoustic Levitational (BAL) Assembly Method for Engineering of Multilayered, 3D Brain-Like Constructs, Using Human Embryonic Stem Cell Derived Neuro-Progenitors. Adv. Mater. Weinheim (2015). doi:10.1002/adma.201503916