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New autocatalytic reaction schemes for molecular amplification in biomolecular diagnostics.
Par François Mavré (Lab. Electrochimie Moléculaire, Université Paris Diderot)
Le 5 Décembre 2017 à 11h00 - Salle de séminaires 5ème étage, Tour 32-33


Biomolecular diagnostic requires high level of selectivity and sensitivity to provide relevant information about the pathological state of a patient. While selectivity relies often on specific supramolecular interactions with a given probe, sensitivity depends on the capacity to generate sufficient signal compared to background noise. Commonly, three general strategies are used: target consumption with signal amplification, target recycling, or target amplification. For instance, PCR is a gold standard bioanalytical technique based on target amplification, in which the targeted sequence catalyzes its own production through a temperature-dependent autocatalytic reaction scheme. Such autocatalytic reactions are of great analytical interest (indeed, PCR allows the detection of only few copies per samples). Alternative autocatalytic reactions have been developed for the isothermal detection of nucleic acids but they again all rely on the abundant bio-molecular machinery available for DNA (polymerase, nuclease, ligase,…). Unfortunately, such universal tools are not available for targets as proteins or small organic/inorganic molecules. Alternative strategies must therefore be developed in order to take advantage of autocatalytic reactions in the detections of such targets. In this talk, I will present our ongoing research on new autocatalytic reaction schemes for target-triggered molecular amplification with application to bio-molecular diagnostic. In particular, we explore systems based on the coupling between a hydrogen peroxide-triggered release of a quinonic compounds and its redox cycling in presence of molecular oxygen and an appropriate reducing agent.