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Seminaire Interne: TBA
Par Hugo Trentesaux / Aurore Woller
Le 3 Mars 2020 à 11h00 - Salle de séminaires 5ème étage, Tour 32-33


Hugo Trentesaux
A two-photon rotating light sheet microscope to study multisensory integration in larval zebrafish:
Zebrafish larva revealed itself to be a good model to study brain-wide dynamics at single-cell level. We previously build a miniaturized rotating light sheet microscope that allowed us to record its neuronal response to vestibular stimulation [Migault 2018]. Preliminary behavioral studies shows that the response to this kind of stimulation depends on the larva's visual environment. To get a good control over it, we used an invisible infrared laser in a two-photon version of the light-sheet microscope [Wolf 2017]. In order to combine these two sensory modalities into a single experiment, we explored the track of making a two-photon-rotating-light-sheet microscope.


Aurore Woller
Immunity and competition between bacteria in the gut:
Antibiotic resistance is a major threat, motivating the development of procedures to eliminate antibiotic resistant bacteria. Our collaborators (E. Slack’s lab, ETH Zürich) have shown that intestinal antibodies, raised by oral vaccines, enforce the targeted bacterial strain to undergo "enchained growth", forming large clumps, which may be flushed out of the gut faster than free bacteria. They are currently developing a protocol combining vaccination with the introduction of a niche competitor to eliminate undesirable strains. We are developing biophysical models using differential equations to quantify the dynamics of this targeted antibiotic-free elimination. We find that whether an undesirable strain can be eliminated from the gut by a niche competitor and how long it takes strongly depends on the growth rates of both strains, on their loss rates, as well as on the carrying capacity. Combining analytical and numerical approaches, we use Salmonella competition data to estimate these kinetic parameters. This allows us to propose an estimation of the time needed to clear a Salmonella strain in the presence of a competitor.