2018
2019
Master 2
Micro-organism network : cooperation and/or competition
Supervisor: Nelly Henry

Context: We are working on communities of microorganisms adhering to surfaces so called 'Biofilms'. They represent the main micro-organism lifestyle.  In there, the community develops a specific functioning whose mechanisms remain to be elucidated. We are interested in understanding the interplay of the physical and physico-chemical interactions within these systems and in how it impacts the main biological functions of the biofilm.

Objective: We propose an internship focused on the study of a 4-species model community of which we characterized the formation of milifluidic system. The aim of the internship is to create a millifluidic network allowing to question the ability of different species to explore the space - individually or in groups - and to decide on the cooperation and competition phenomena that appear between the 4 species in a branched physical network. The objective is to highlight the factors that dominate the assembly of multi-species bacterial communities, potentially: resistance to flow, motility, adhesive capacity, adaptation to nutrient depletion...

Practically: This work involves the design of the physical network, its realization in PDMS casts, the characterization of its hydrodynamics, its colonization by the bacterial 4-species consortium and its monitoring in video fluorescence microscopy. The information will emerge from implementing image analysis protocols. This M2 internship will be closely coupled with an ongoing thesis work, dedicated to the analysis of disturbances of the 4-species community, which has already enable to settle all the technical issues associated with real time monitoring of living biofilms in millifluidic geometries.

Profile sought: Physicist or physico-chemist attracted by biological questions, or biologist trained in quantitative analyses and data processing

 

 

 

Related publications

Intermittent Pili-Mediated Forces Fluidize Neisseria meningitidis Aggregates Promoting Vascular Colonization - Cell
D. Bonazzi , V. Lo Schiavo , S. Machata , I. Djafer-Cherif , P. Nivoit , V. Manriquez , H. Tanimoto , J. Husson , N. Henry , H. Chate , R. Voituriez , G. Dumenil
  URL Full text PDF Bibtex doi:10.1016/j.cell.2018.04.010
The inducible chemical-genetic fluorescent marker FAST outperforms classical fluorescent proteins in the quantitative reporting of bacterial biofilm dynamics - Scientific Reports
A. Monmeyran , P. Thomen , H. Jonquiere , F. Sureau , C. Li , M.a. Plamont , c. Douarche , j.F. Casella , A. Gautier , N. Henry
  URL Full text PDF Bibtex doi:10.1038/s41598-018-28643-z

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