2019
2020
Master 2
Multi-species biofilm dynamics
Supervisor: Nelly Henry

Bacterial biofilm dynamics

(contact: nelly.henry@upmc.fr)

Context: We are working on communities of microorganisms adhering to surfaces so called 'Biofilms'. They represent the main micro-organism lifestyle.  In there, the community develops a specific functioning whose mechanisms remain to be elucidated. We are interested in understanding the interplay of the physical and physico-chemical interactions within these systems and in how it impacts the main biological functions of the biofilm. Several aspects are currently under study in the group. We explore

- The internal dynamics of these communities either by using colloidal probes, or by using the cells themselves as a probe
- The spatio-temporal mapping of oxygen in these biofilms
- The kinetics of formation of multi-species communities addressing the question of which intersepcies interactions emerge and how do they appear
- The effects of light on bacterial biofilms

 

M2 internship: The M2 internship will be a contribution to one of these themes. It will allow the trainee to learn the main methods common to all these approaches: basic microbiology, microfabrication, millifluidic, fluorescence video-microscopy and/or fluorescence lifetime, image analysis, development of 'data analysis routines.

Potentially, the internship will continue with a PhD in the context of a European Fet-Open contract focusing on the effects of light on bacterial biofilms.

Profile sought: Physicist or physico-chemist attracted by biological questions, or biologist trained in quantitative analysis and data processing. Operational knowledge in Matlab is preferable.

 

 

 

Related publications

Intermittent Pili-Mediated Forces Fluidize Neisseria meningitidis Aggregates Promoting Vascular Colonization - Cell
D. Bonazzi , V. Lo Schiavo , S. Machata , I. Djafer-Cherif , P. Nivoit , V. Manriquez , H. Tanimoto , J. Husson , N. Henry , H. Chate , R. Voituriez , G. Dumenil
  URL Full text PDF Bibtex doi:10.1016/j.cell.2018.04.010
The inducible chemical-genetic fluorescent marker FAST outperforms classical fluorescent proteins in the quantitative reporting of bacterial biofilm dynamics - Scientific Reports
A. Monmeyran , P. Thomen , H. Jonquiere , F. Sureau , C. Li , M.a. Plamont , c. Douarche , j.F. Casella , A. Gautier , N. Henry
  URL Full text PDF Bibtex doi:10.1038/s41598-018-28643-z

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