Toward nonlinear endomicroscopy for exploration of the pulmonary airways: preliminary spectroscopic study of human lung tissue using a single-mode fibre

D.A. Peyrot , C. Lefort , D.A. Haidar , T. Mansuryan , S. Kruglik , N. Sandeau , F. Louradour , G. Bourg-Heckly

Bibtex , URL
ENDOSCOPIC MICROSCOPY VI, 7893, 789309 (2011)
DOI: 10.1117/12.875440
ISSN: 0277-786X


We are developing a non-linear fibered endomicroscope for imaging the extracellular matrix collagen and elastin fibrillar networks during bronchoscopy. As a proof of concept, laser pulses at the output of a standard 2 meter long single-mode fibre have been obtained with pulse duration of about 50 fs and pulse energy up to 50 uJ, using a specially designed grism line for the dispersion compensation. With these pulses, we performed a spectroscopic characterization of the non-linear endogenous signal, consisting of two-photon fluorescence and second harmonic generation, and originated from human pulmonary tissue of various thickness, both in forward and backward geometry of signal collection, with excitation at 830 nm.

Cette publication est associée à :

Biophotonique : Spectro-imagerie à visée diagnostique