ACA-Pro: calibration protocol for quantitative diffuse reflectance spectroscopy. Validation on contact and noncontact probe- and CCD-based systems

V. Sorgato , M. Berger , C. Emain , C. Vever-Bizet , J.M. Dinten , G. Bourg-Heckly , A. Planat-Chretien

Bibtex , URL
J. Biomed. Opt., 21, 6, 065003.1 - 065003.9
Published 01 Jun. 2016
DOI: 10.1117/1.JBO.21.6.065003
ISSN: 1083-3668


We have developed an adaptive calibration algorithm and protocol (ACA-Pro) that corrects from the instrumental response of various spatially resolved diffuse reflectance spectroscopy (DRSsr) systems to enable the quantification of absorption and scattering properties based on a Monte Carlo-based look-up-table approach. The protocol involves the use of a calibration reference base built with measurements of a range of different diffusive intralipid phantoms. Moreover, an advanced strategy was established to take into account the experimental variations with an additional measurement of a common solid material, allowing the use of a single calibration reference base for all experiments. The ACA-Pro is validated in contact and noncontact probe-based DRSsr systems. Furthermore, the first results of a setup replacing the probe with a CCD detector are shown to confirm the robustness of the approach. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE).

Cette publication est associée à :

Biophotonique : Spectro-imagerie à visée diagnostique