Spectral and lifetime domain measurements of rat brain tumours

H.D. Abi , B. Leh , K. Allaoua , A. Genoux , R. Siebert , M. Steffenhagen , D. Peyrot , N. Sandeau , C. Vever-Bizet , G. Bourg-Heckly , I. Chebbi , M. Collado-Hilly

Bibtex , URL
PHOTONIC THERAPEUTICS AND DIAGNOSTICS VIII, PTS 1 AND 2, 8207, 82074P (2012)
DOI: 10.1117/12.907411
ISSN: 0277-786X

Abstract

During glioblastoma surgery, delineation of the brain tumour margins remains difficult especially since infiltrated and normal tissues have the same visual appearance. This problematic constitutes our research interest. We developed a fibre-optical fluorescence probe for spectroscopic and time domain measurements. First measurements of endogenous tissue fluorescence were performed on fresh and fixed rat tumour brain slices. Spectral characteristics, fluorescence redox ratios and fluorescence lifetime measurements were analysed. Fluorescence information collected from both, lifetime and spectroscopic experiments, appeared promising for tumour tissue discrimination. Two photon measurements were performed on the same fixed tissue. Different wavelengths are used to acquire two-photon excitation-fluorescence of tumorous and healthy sites.

Cette publication est associée à :

Biophotonique : Spectro-imagerie à visée diagnostique